Laboratory for Molecular Delivery and Imaging Technology | RIKEN BDR

Laboratory for Molecular Delivery and Imaging Technology

Team Leader

Hidefumi MukaiPh.D.

Photo of principal investigator

  • Location:Kobe / MI R&D Center Building
  • E-mail:hmukai[at]riken.jpPlease replace [at] with @.

Targeting the control molecules to the specific organs and their target cells by elucidation of better dynamics in the whole body.

Research Summary

The objective of our team is to develop evaluation and verification technologies of the function of control molecules in situ and in vivo, and to develop an efficient Drug Delivery System (DDS).

Research Theme

  • Development of bacterial machines for cancer therapy
  • Biomacromolecular PET probes: ADME studies and imaging biomarker development
  • Application of inorganic- and bio-nanoparticles to DDS

Main Publications List

  • Nomura S, Takahashi M, Hashiba Kato A, et al.
    Biosorption-based 64Cu-labeling of bacteria for pharmacokinetic positron-emission tomography.
    International Journal of Pharmaceutics 590. 119950 (2020) doi:10.1016/j.ijpharm.2020.119950
  • Mukai H, Watanabe Y.
    Review: PET imaging with macro- and middle-sized molecular probes.
    Nuclear Medicine and Biology, in press doi:10.1016/j.nucmedbio.2020.06.007
  • Sakai K, Passioura T, Sato H, et al.
    Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor.
    Nature Chemical Biology 15. 598–606 (2019) doi: 10.1038/s41589-019-0285-7
  • Ren Q, Mohri K, Warashina S, et al.
    Improved immuno-PET imaging of HER2-positive tumors in mice: Urokinase injection-triggered clearance enhancement of 64Cu-trastuzumab.
    Molecular Pharmaceutics 16(3). 1065-1073 (2019) doi: 10.1021/acs.molpharmaceut.8b01052
  • Mukai H, Hatanaka K, Yagi N, et al.
    Pharmacokinetic evaluation of liposomal nanoparticle-encapsulated nucleic acid drug: A combined study of dynamic PET imaging and LC/MS/MS analysis.
    Journal of Controlled Release 294. 185-194 (2019) doi: 10.1016/j.jconrel.2018.12.006
  • Nakamoto Y, Pradipta AR, Mukai H, et al.
    Expanding the applicability of the metal labeling of biomolecules by the RIKEN click reaction: A case study with Gallium‐68 positron emission tomography.
    Chembiochem 19. 2055-2060 (2018) doi: 10.1002/cbic.201800335
  • Matsumura K, Zouda M, Wada Y, et al.
    Urokinase injection-triggered clearance enhancement of a 4-arm PEG-conjugated 64Cu-bombesin analog tetramer: A novel approach for the improvement of PET imaging contrast.
    International Journal of Pharmaceutics 545. 206-214 (2018) doi:10.1016/j.ijpharm.2018.05.014
  • Mukai H, Takahashi M, Watanabe Y.
    Potential usefulness of Brevibacillus for bacterial cancer therapy: intratumoral provision of tumor necrosis factor- α and anticancer effects.
    Cancer Gene Therapy 25. 47-57 (2018) doi:10.1038/s41417-017-0009-7
  • Otani Y, Kawakami S, Mukai H, et al.
    Long-term in vivo gene expression in mouse kidney using φ31 integrase and electroporation.
    Journal of Drug Targeting 23. 427-435 (2015) doi:10.3109/1061186X.2014.1002788
  • Mukai H, Ozaki D, Cui YL, et al.
    Quantitative evaluation of the improvement in the pharmacokinetics of a nucleic acid drug delivery system by dynamic PET imaging with 18 F-incorporated oligodeoxynucleotides.
    Journal of Controlled Release 180. 92-99 (2014) doi:10.1016/j.jconrel.2014.02.014
  • Mukai H, Wada Y, Watanabe Y.
    The synthesis of 64 Cu-chelated porphyrin photosensitizers and their tumor-targeting peptide conjugates for the evaluation of target cell uptake and PET image-based pharmacokinetics of targeted photodynamic therapy agents.
    Annals of Nuclear Medicine 27. 625-639 (2013) doi:10.1007/s12149-013-0728-2
  • Mukai H, Kawakami S, Takahashi H, et al.
    Key physiological phenomena governing transgene expression based on tissue pressure-mediated transfection in mice.
    Biological and Pharmaceutical Bulletin 33. 1627-1632 (2010) doi:10.1248/bpb.33.1627
  • Mukai H, Kawakami S, Kamiya Y, et al.
    Pressure-mediated transfection of murine spleen and liver.
    Human Gene Therapy 20. 1157-1167 (2009) doi:10.1089/hum.2008.213
  • Ohyama J, Hitomi Y, Higuchi Y, et al.
    One-Phase Synthesis of Small Gold Nanoparticles Coated by a Horizontal Porphyrin Monolayer.
    Chemical Communications 47. 6300-6302 (2008) doi:10.1039/b815100h


Hidefumi MukaiTeam Leader hmukai[at]
Kohta MohriResearch Scientist kota.mori[at]
Qin RenResearch Scientist[at]
Shota WarashinaResearch Scientist
Maiko TakahashiResearch Associate mtakahashi[at]
Shoko NomuraPostdoctoral Researcher
Maki ZoudaTechnical Staff I maki.zouda[at]
Erike Widyasari SukowatiTechnical Staff I
Chizuru KiyotaTemporary Staffing
Akari HashibaStudent Trainee
Yoko NagatomoAssistant*

*:concurrent / Please replace [at] with @.