Laboratory for Nano-Bio Probes | RIKEN BDR

Laboratory for Nano-Bio Probes

Team Leader

Takashi JinD.Sci.

Photo of principal investigator

  • Location:Osaka / Quantitative Biology Buildings
  • E-mail:tjin[at]riken.jpPlease replace [at] with @.

Development of near-infrared bio probes for non-invasive molecular imaging

Research Summary

Our research team develops novel nano-bio probes for visualization of molecular and cellular dynamics in biological systems. We have synthesized a variety of nano-bio probes such as single-molecule imaging probes using quantum dots, cellular environment sensitive probes, and cellular state controllable probes using external magnetic fields. Recently, we have developed deep-tissue imaging probes to visualize cellular dynamics in vivo. We synthesize multi-colored fluorescent probes that emit NIR light (1000-1400 nm; the second optical window in living tissues), using cyanine dyes, fluorescent proteins, quantum dots, and rare-earth ions. By using the NIR fluorescent probes, we will visualize cancer metastasis and immune reactions at the cellular level in living systems. We are also developing probe delivery techniques (using DDS) for targeting cancer tissues and organs in small animals.

Minute difference in size of Quantum dots produces different fluorescent colors

Cell surface proteins visualized in multi-color by Quantum dots

Research Theme

  • Multi-colored single-molecule imaging probes with high brightness and photo-stability
  • Cellular-environment sensitive near-infrared probes that respond to membrane potential, pH, and solvent polarity
  • Bioluminescence-coupled self illuminating near-infrared probes
  • Near-infrared probes that can be used in the second optical window (1000-1500 nm)
  • In vivo fluorescence imaging of cancer metastasis, immune/ inflammation responses at the cellular level

Main Publications List

  • Swamy MMM, Murai Y, Monde K, et al.
    Shortwave-Infrared Fluorescent Molecular Imaging Probes Based on π-Conjugation Extended Indocyanine Green.
    Bioconjugate Chemistry (2021) doi: 10.1021/acs.bioconjchem.1c00253
  • Tsuboi S, Jin T.
    Dual-colour (near-infrared/visible) emitting annexin V for fluorescence imaging of tumour cell apoptosis in vitro and in vivo.
    RSC Advances 10. 38244-38250 (2020) doi: 10.1039/d0ra06495e
  • Tiwari D.K, Tiwari M, Jin T.
    Near-infrared fluorescent protein and bioluminescence-based probes for high-resolution in vivo optical imaging.
    Materials Advances 1. 967-987 (2020) doi:10.1039/d0ma00273a
  • Tsuboi S, Jin T.
    Shortwave-infrared (SWIR) fluorescence molecular imaging using indocyanine green-antibody conjugates for the optical diagnostics of cancerous tumours.
    RSC Advances 10. 28171–28179 (2020) doi: 10.1039/d0ra04710d
  • Tsuboi S, Jin T.
    BRET-based dual-color (visible/near-infrared) molecular imaging using a quantum dot/EGFP-luciferase conjugate.
    RSC Advances 9. 34964-34971 (2019) doi: 10.1039/c9ra07011g
  • Jin T.
    Recent progress in NIR fluorophores emitting over 1000 nm for bioimaging.
    ECS Journal of Solid State Science and Technology 8. R9-R13 (2019) doi: 10.1149/2.0111901jss
  • Tsuboi S, Jin T.
    Fluorescent recombinant protein conjugated near-infrared emitting quantum dots for in vitro and in vivo dual-color molecular imaging.
    ChemBioChem 20. 565-575 (2019) doi: 10.1002/cbic.201800506
  • Tsuboi S, Jin T.
    Recombinant Protein (Luciferase-IgG Binding Domain) Conjugated Quantum Dots for BRET-Coupled Near-Infrared Imaging of Epidermal Growth Factor Receptors.
    Bioconjugate Chemistry 29(4). 1466-1574 (2018) doi: 10.1021/acs.bioconjchem.8b00149
  • Tsuboi S, Yamada S, Nakane Y, Sakata T, et al.
    Water-soluble near-infrared fluorophores emitting over 1000 nm and their application to in vivo imaging in the second optical window (1000-1400nm)
    ECS Journal of Solid State Science and Technology 7(1). 3093-3101 (2018) doi: 10.1149/2.0141801jss
  • Tsuboi S, Sasaki A, Sakata T, Yasuda H et al.
    Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging.
    Chemical Communications 53. 9450-9453 (2017) doi: 10.1039/c7cc04966h
  • Tsuboi S and Jin T.
    Bioluminescence Resonance Energy Transfer (BRET)-coupled Annexin V-functionalized Quantum Dots for Near-Infrared Optical Detection of Apoptotic Cells.
    ChemBioChem 18(22).2231-2235(2017) doi: 10.1002/cbic.201700486